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MB 452 Microbial Diversity Lab
This lab course is project-oriented. Students perform a series of classical enrichments and isolations, starting from environmental samples collected by the students themselves. Some of these isolations serve as the starting materials for a series of modern molecular biology experiments, in which students purify DNA, amplify ribosomal DNA by PCR, and have a portion of this gene sequenced. This sequence information is the starting point for the term project, a detailed molecular phylogenetic analysis of the isolated organisms. Students will be required to provide their own transportation during non-scheduled class time for local field sample collection.
Prerequisite: MB 412; Corequisite: ²ÑµþÌý451
Typically offered in Fall only